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Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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maldi tof mass spectrometer  (Bruker Corporation)
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Bruker Corporation maldi tof mass spectrometer
Peptide barcode analysis of CH-negative and CH-positive samples <t>via</t> <t>MALDI-TOF</t> MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).
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Peptide barcode analysis of CH-negative and CH-positive samples via MALDI-TOF MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).

Journal: Scientific Reports

Article Title: Rapid peptide analysis in dried bloodspots to identify novel markers for newborn screening for congenital hypothyroidism

doi: 10.1038/s41598-026-42578-w

Figure Lengend Snippet: Peptide barcode analysis of CH-negative and CH-positive samples via MALDI-TOF MS. ( a ) Representative MALDI-TOF MS spectra comparing CH-negative and CH-positive patients within the range of 1,000–6,000; a total of 1,434 peptide features were detected. ( b ) Hierarchical heatmap visualization of the peptide barcode. Relative expression levels are represented by color intensity, where red denotes high expression and blue denotes low expression. The heatmap was generated using MetaboAnalyst 6.0 ( https://www.metaboanalyst.ca/ ).

Article Snippet: MALDI-TOF MS spectra were acquired using a JMS-S3000 SpiralTOF mass spectrometer (JEOL Ltd., Japan) operating in linear positive-ion mode.

Techniques: Expressing, Generated